Excision of the Conjugative Transposon Tn 916 in Lactococcus lactis
نویسندگان
چکیده
منابع مشابه
Regulation of excision genes of the Bacteroides conjugative transposon CTnDOT.
The first step in the transfer of the Bacteroides conjugative transposon CTnDOT is excision of the integrated element from the chromosome to form a circular transfer intermediate. Excision occurs only after the bacteria are exposed to tetracycline. Previously, four excision genes were identified. One was the integrase gene intDOT, which appeared to be expressed constitutively. Three other genes...
متن کاملIntegration and excision of a Bacteroides conjugative transposon, CTnDOT.
Bacteroides conjugative transposons (CTns) are thought to transfer by first excising themselves from the chromosome to form a nonreplicating circle, which is then transferred by conjugation to a recipient. Earlier studies showed that transfer of most Bacteroides CTns is stimulated by tetracycline, but it was not known which step in transfer is regulated. We have cloned and sequenced both ends o...
متن کاملInsertion of Transposon Tn917 Derivatives into the Lactococcus lactis subsp. lactis Chromosome.
Two transposition vectors, pTV32 and pLTV1, containing transposon Tn917 derivatives TV32 and LTV1, respectively, were introduced into Lactococcus lactis subsp. lactis MG1614. It was found that pTV32 and pLTV1 replicate and that TV32 and LTV1 transpose in this strain. A protocol for production of a collection of Tn917 insertions in L. lactis subsp. lactis was developed. The physical locations of...
متن کاملCloning of EprA1 gene of Aeromonas hydrophila in Lactococcus lactis
Bacterial-based systems as live vectors for the delivery of heterologous antigens offer a number of advantages as vaccination strategies. Developments in genetic engineering have given Gram-positive lacticacid bacteria (LAB) the advantage of being used as a host expression system for antigen delivery to inducethe immune response. A fragment containing the full length of the “eprA1” ...
متن کاملExcision of a conjugative transposon in vitro by the Int and Xis proteins of Tn916.
The roles of purified Int and Xis proteins of the conjugative transposon Tn 916 in excision of a deletion derivative of the closely related element Tn 1545 were investigated. At a low salt concentration (37.5 mM NaCl), Int alone was able to promote limited excision to produce a covalently closed circular form of the transposon, showing that Tn 916 Int can catalyze both DNA cleavage and strand e...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1999
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.65.5.2230-2231.1999